Review



androgen independent du145and pc3 human tumor prostate cell lines  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC androgen independent du145and pc3 human tumor prostate cell lines
    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with <t>PC3</t> cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.
    Androgen Independent Du145and Pc3 Human Tumor Prostate Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/androgen independent du145and pc3 human tumor prostate cell lines/product/ATCC
    Average 99 stars, based on 15756 article reviews
    androgen independent du145and pc3 human tumor prostate cell lines - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Dissecting the novel molecular interactions of solute carrier family 4 member 4 (SLC4A4) for prostate cancer (PCa) progression"

    Article Title: Dissecting the novel molecular interactions of solute carrier family 4 member 4 (SLC4A4) for prostate cancer (PCa) progression

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-72408-w

    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with PC3 cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.
    Figure Legend Snippet: Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with PC3 cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.

    Techniques Used: Expressing, Immunohistochemical staining, Over Expression, Western Blot



    Similar Products

    androgen independent du145and pc3 human tumor prostate cell lines  (ATCC)
    99
    ATCC androgen independent du145and pc3 human tumor prostate cell lines
    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with <t>PC3</t> cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.
    Androgen Independent Du145and Pc3 Human Tumor Prostate Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/androgen independent du145and pc3 human tumor prostate cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
    androgen independent du145and pc3 human tumor prostate cell lines - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    pc3 human prostate tumor cell lines  (ATCC)
    99
    ATCC pc3 human prostate tumor cell lines
    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with <t>PC3</t> cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.
    Pc3 Human Prostate Tumor Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pc3 human prostate tumor cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
    pc3 human prostate tumor cell lines - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    pc3 human tumor prostate cell lines  (ATCC)
    99
    ATCC pc3 human tumor prostate cell lines
    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with <t>PC3</t> cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.
    Pc3 Human Tumor Prostate Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pc3 human tumor prostate cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
    pc3 human tumor prostate cell lines - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    human prostate tumor cell lines pc3  (DSMZ)
    94
    DSMZ human prostate tumor cell lines pc3
    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with <t>PC3</t> cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.
    Human Prostate Tumor Cell Lines Pc3, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human prostate tumor cell lines pc3/product/DSMZ
    Average 94 stars, based on 1 article reviews
    human prostate tumor cell lines pc3 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    human prostate tumor pc3 cell line  (ATCC)
    99
    ATCC human prostate tumor pc3 cell line
    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with <t>PC3</t> cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.
    Human Prostate Tumor Pc3 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human prostate tumor pc3 cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    human prostate tumor pc3 cell line - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    human prostate tumor cell line pc3  (DSMZ)
    94
    DSMZ human prostate tumor cell line pc3
    Adhesion of temsirolimus (TEM)-resistant <t>(PC3</t> res ) and TEM-sensitive (PC3 par ) prostate cancer cells. ( A ) time-dependent PC3 adhesion to human vein endothelial cells (HUVEC); ( B ) binding to immobilized collagen; ( C ) binding to immobilized fibronectin; ( D ) binding to immobilized laminin. * indicates significant difference to the temsirolimus-free control (PC3 par ). # indicates significant difference between cells exposed to 10 nm of TEM and untreated cells.
    Human Prostate Tumor Cell Line Pc3, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human prostate tumor cell line pc3/product/DSMZ
    Average 94 stars, based on 1 article reviews
    human prostate tumor cell line pc3 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with PC3 cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.

    Journal: Scientific Reports

    Article Title: Dissecting the novel molecular interactions of solute carrier family 4 member 4 (SLC4A4) for prostate cancer (PCa) progression

    doi: 10.1038/s41598-024-72408-w

    Figure Lengend Snippet: Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with PC3 cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.

    Article Snippet: Androgen-dependent 22RV1, LNCAP, VCAP, C4-2 and androgen-independent DU145and PC3 human tumor prostate cell lines were purchased from the American Type Culture Collection (ATCC).

    Techniques: Expressing, Immunohistochemical staining, Over Expression, Western Blot

    Adhesion of temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. ( A ) time-dependent PC3 adhesion to human vein endothelial cells (HUVEC); ( B ) binding to immobilized collagen; ( C ) binding to immobilized fibronectin; ( D ) binding to immobilized laminin. * indicates significant difference to the temsirolimus-free control (PC3 par ). # indicates significant difference between cells exposed to 10 nm of TEM and untreated cells.

    Journal: Cells

    Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

    doi: 10.3390/cells7090129

    Figure Lengend Snippet: Adhesion of temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. ( A ) time-dependent PC3 adhesion to human vein endothelial cells (HUVEC); ( B ) binding to immobilized collagen; ( C ) binding to immobilized fibronectin; ( D ) binding to immobilized laminin. * indicates significant difference to the temsirolimus-free control (PC3 par ). # indicates significant difference between cells exposed to 10 nm of TEM and untreated cells.

    Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

    Techniques: Binding Assay, Control

    Motility of temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. Mean values were calculated from five counts. ( A ) PC3 chemotaxis; ( B ) PC3 migration; ( C ) PC3 invasion. * indicates significant difference to the temsirolimus-free control (PC3 par ). # indicates significant difference between the treated and untreated PC3 sublines with 10 nm TEM.

    Journal: Cells

    Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

    doi: 10.3390/cells7090129

    Figure Lengend Snippet: Motility of temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. Mean values were calculated from five counts. ( A ) PC3 chemotaxis; ( B ) PC3 migration; ( C ) PC3 invasion. * indicates significant difference to the temsirolimus-free control (PC3 par ). # indicates significant difference between the treated and untreated PC3 sublines with 10 nm TEM.

    Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

    Techniques: Chemotaxis Assay, Migration, Control

    Flow activated cell sorting (FACS) analysis of integrin α and β subtype expression on temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. Mean fluorescence values are shown below the histograms (Par = PC3 parental cells, Res = PC3-resistant cells). One of three independent experiments.

    Journal: Cells

    Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

    doi: 10.3390/cells7090129

    Figure Lengend Snippet: Flow activated cell sorting (FACS) analysis of integrin α and β subtype expression on temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. Mean fluorescence values are shown below the histograms (Par = PC3 parental cells, Res = PC3-resistant cells). One of three independent experiments.

    Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

    Techniques: FACS, Expressing, Fluorescence

    ( A ) Integrin protein level in temsirolimus-resistant (PC3 res ) versus temsirolimus-sensitive (PC3 par ) cells quantified by pixel density analysis. Integrins were evaluated three times, integrin-linked kinase (ILK), focal adhesion kinase (FAK), and phosphorylated focal adhesion kinase (pFAK) four times. Representative Western blots are shown on the right panel. ( B ) The integrin gene expression pattern in PC3 res versus PC3 par cells. Values are given as fold difference to PC3 par cells. * indicates a significant difference.

    Journal: Cells

    Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

    doi: 10.3390/cells7090129

    Figure Lengend Snippet: ( A ) Integrin protein level in temsirolimus-resistant (PC3 res ) versus temsirolimus-sensitive (PC3 par ) cells quantified by pixel density analysis. Integrins were evaluated three times, integrin-linked kinase (ILK), focal adhesion kinase (FAK), and phosphorylated focal adhesion kinase (pFAK) four times. Representative Western blots are shown on the right panel. ( B ) The integrin gene expression pattern in PC3 res versus PC3 par cells. Values are given as fold difference to PC3 par cells. * indicates a significant difference.

    Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

    Techniques: Western Blot, Gene Expression

    Influence of integrin α2, α5, or β1 blockade on PC3 adhesion, chemotaxis, and migration. Values are shown as percentage difference to their respective 100% controls. * indicates significant difference between the PC3 control subline and the PC3 subline treated with the function-blocking antibody. # indicates significant difference between temsirolimus-sensitive (PC3 par ) and temsirolimus-resistant (PC3 res ) cells whose integrin subtype was blocked.

    Journal: Cells

    Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

    doi: 10.3390/cells7090129

    Figure Lengend Snippet: Influence of integrin α2, α5, or β1 blockade on PC3 adhesion, chemotaxis, and migration. Values are shown as percentage difference to their respective 100% controls. * indicates significant difference between the PC3 control subline and the PC3 subline treated with the function-blocking antibody. # indicates significant difference between temsirolimus-sensitive (PC3 par ) and temsirolimus-resistant (PC3 res ) cells whose integrin subtype was blocked.

    Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

    Techniques: Chemotaxis Assay, Migration, Control, Blocking Assay

    Adhesion of temsirolimus (TEM)-resistant (PC3 res ) versus TEM-sensitive (PC3 par ) prostate cancer cells in the presence of valproic acid (VPA). The figure depicts time-dependent PC3 adhesion to human umbilical vein endothelial cells (HUVEC), binding to immobilized collagen, fibronectin, or laminin. * indicates significant difference to controls not treated with VPA.

    Journal: Cells

    Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

    doi: 10.3390/cells7090129

    Figure Lengend Snippet: Adhesion of temsirolimus (TEM)-resistant (PC3 res ) versus TEM-sensitive (PC3 par ) prostate cancer cells in the presence of valproic acid (VPA). The figure depicts time-dependent PC3 adhesion to human umbilical vein endothelial cells (HUVEC), binding to immobilized collagen, fibronectin, or laminin. * indicates significant difference to controls not treated with VPA.

    Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

    Techniques: Binding Assay

    ( A , B ). Chemotactic movement and migration of PC3 res versus PC3 par cells treated with valproic acid (VPA). Values are given as percentage difference to their respective 100% controls. * indicates significant difference to controls not treated with VPA. ( C ). Influence of VPA on integrin α2, α5, or β1 expression. Mean fluorescence units (MFU) are shown as percentage difference to the respective 100% controls (not treated with VPA). ( D ) Influence of VPA on Akt expression. Akt and pAkt levels were quantified by Western blotting and pixel density analysis. Pixel density values of the pAkt/Akt ratio and representative Western blots are shown (Ctrl = control). * indicates significant difference between the PC3 control subline and the PC3 subline treated with VPA. # indicates significant difference between PC3 par and PC3 res cells.

    Journal: Cells

    Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

    doi: 10.3390/cells7090129

    Figure Lengend Snippet: ( A , B ). Chemotactic movement and migration of PC3 res versus PC3 par cells treated with valproic acid (VPA). Values are given as percentage difference to their respective 100% controls. * indicates significant difference to controls not treated with VPA. ( C ). Influence of VPA on integrin α2, α5, or β1 expression. Mean fluorescence units (MFU) are shown as percentage difference to the respective 100% controls (not treated with VPA). ( D ) Influence of VPA on Akt expression. Akt and pAkt levels were quantified by Western blotting and pixel density analysis. Pixel density values of the pAkt/Akt ratio and representative Western blots are shown (Ctrl = control). * indicates significant difference between the PC3 control subline and the PC3 subline treated with VPA. # indicates significant difference between PC3 par and PC3 res cells.

    Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

    Techniques: Migration, Expressing, Fluorescence, Western Blot, Control